Anti-MUC16 antibody (12660-12923) (STJ11105388)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Mucin-16 (12660-12923) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MUC16 |
| Gene ID | 94025 |
| Uniprot ID | MUC16_HUMAN |
| Immunogen Region | 12660-12923 |
| Immunogen Sequence | GFTHWIPVPTSSTPGTSTVD LGSGTPSSLPSPTTAGPLLV PFTLNFTITNLKYEEDMHCP GSRKFNTTERVLQSLLGPMF KNTSVGPLYSGCRLTLLRSE KDGAATGVDAICTHRLDPKS PGVDREQLYWELSQLTNGIK ELGPYTLDRNSLYVNGFTHQ TSAPNTSTPGTSTVDLGTSG TPSSLPSPTSAGPLLVPFTL NFTITNLQYEEDMHHPGSRK FNTTERVLQGLLGPMFKNT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 12660-12923 of human CA125 (NP_078966.2). |
Additional Info
| Tissue Specificity | Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. |
| Post Translational Modifications | Heavily O-glycosylated.expresses both type 1 and type 2 core glycans. Heavily N-glycosylated.expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
| Function | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. |
| Protein Name | Mucin-16Muc-16Ovarian Cancer-Related Tumor Marker Ca125Ca-125Ovarian Carcinoma Antigen Ca125 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSecretedExtracellular SpaceMay Be Liberated Into The Extracellular Space Following The Phosphorylation Of The Intracellular C-Terminus Which Induces The Proteolytic Cleavage And Liberation Of The Extracellular Domain |
| Alternative Antibody Names | Anti-Mucin-16 antibodyAnti-Muc-16 antibodyAnti-Ovarian Cancer-Related Tumor Marker Ca125 antibodyAnti-Ca-125 antibodyAnti-Ovarian Carcinoma Antigen Ca125 antibodyAnti-MUC16 antibodyAnti-CA125 antibody |
Information sourced from Uniprot.org