• Anti-MUC1 antibody [MRQ-17] (STJ16100397)

Anti-MUC1 antibody [MRQ-17] (STJ16100397)

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STJ16100397

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-MUC1 is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: MRQ-17
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Dilution Range: IHC 1:50 – 1:200
Storage Instruction: Store at 2-8°C upon receipt.
Gene Symbol: MUC1
Gene ID: 4582
Uniprot ID: MUC1_HUMAN
Tissue Specificity Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
Post Translational Modifications Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM. Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17. Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane. Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1. The N-terminal sequence has been shown to begin at position 24 or 28.
Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Protein Name Mucin-1
Muc-1
Breast Carcinoma-Associated Antigen Df3
Cancer Antigen 15-3
Ca 15-3
Carcinoma-Associated Mucin
Episialin
H23ag
Krebs Von Den Lungen-6
Kl-6
Pemt
Peanut-Reactive Urinary Mucin
Pum
Polymorphic Epithelial Mucin
Pem
Tumor-Associated Epithelial Membrane Antigen
Ema
Tumor-Associated Mucin
Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha
Muc1-Nt
Muc1-Alpha - Mucin-1 Subunit Beta
Muc1-Beta
Muc1-Ct
Database Links Reactome: R-HSA-5083625
Reactome: R-HSA-5083632
Reactome: R-HSA-5083636
Reactome: R-HSA-5621480
Reactome: R-HSA-6785807
Reactome: R-HSA-913709
Reactome: R-HSA-977068
Cellular Localisation Apical Cell Membrane
Single-Pass Type I Membrane Protein
Exclusively Located In The Apical Domain Of The Plasma Membrane Of Highly Polarized Epithelial Cells
After Endocytosis
Internalized And Recycled To The Cell Membrane
Located To Microvilli And To The Tips Of Long Filopodial Protusions
Isoform 5: Secreted
Isoform Y: Secreted
Isoform 9: Secreted
Mucin-1 Subunit Beta: Cell Membrane
Cytoplasm
Nucleus
On Egf And Pdgfrb Stimulation
Transported To The Nucleus Through Interaction With Ctnnb1
A Process Which Is Stimulated By Phosphorylation
On Hrg Stimulation
Colocalizes With Jup/Gamma-Catenin At The Nucleus
Alternative Antibody Names Anti-Mucin-1 antibody
Anti-Muc-1 antibody
Anti-Breast Carcinoma-Associated Antigen Df3 antibody
Anti-Cancer Antigen 15-3 antibody
Anti-Ca 15-3 antibody
Anti-Carcinoma-Associated Mucin antibody
Anti-Episialin antibody
Anti-H23ag antibody
Anti-Krebs Von Den Lungen-6 antibody
Anti-Kl-6 antibody
Anti-Pemt antibody
Anti-Peanut-Reactive Urinary Mucin antibody
Anti-Pum antibody
Anti-Polymorphic Epithelial Mucin antibody
Anti-Pem antibody
Anti-Tumor-Associated Epithelial Membrane Antigen antibody
Anti-Ema antibody
Anti-Tumor-Associated Mucin antibody
Anti-Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha antibody
Anti-Muc1-Nt antibody
Anti-Muc1-Alpha - Mucin-1 Subunit Beta antibody
Anti-Muc1-Beta antibody
Anti-Muc1-Ct antibody
Anti-MUC1 antibody
Anti-PUM antibody

Information sourced from Uniprot.org

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