• Western blot analysis of extracts of mouse thymus, using MTM1 antibody (STJ115478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MTM1 antibody (484-603) (STJ115478)

SKU:
STJ115478

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTM1 (484-603) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTM1
Gene ID: 4534
Uniprot ID: MTM1_HUMAN
Immunogen Region: 484-603
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 484-603 of human MTM1 (NP_000243.1).
Immunogen Sequence: SARERQKVTERTVSLWSLIN SNKEKFKNPFYTKEINRVLY PVASMRHLELWVNYYIRWNP RIKQQQPNPVEQRYMELLAL RDEYIKRLEELQLANSAKLS DPPTSPSSPSQMMPHVQTHF
Function Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis. In skeletal muscles, stabilizes MTMR12 protein levels.
Protein Name Myotubularin
Phosphatidylinositol-3 -5-Bisphosphate 3-Phosphatase
Phosphatidylinositol-3-Phosphate Phosphatase
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660516
Reactome: R-HSA-1660517
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cell Projection
Filopodium
Ruffle
Late Endosome
Myofibril
Sarcomere
Localizes As A Dense Cytoplasmic Network
Also Localizes To The Plasma Membrane
Including Plasma Membrane Extensions Such As Filopodia And Ruffles
Predominantly Located In The Cytoplasm Following Interaction With Mtmr12
Recruited To The Late Endosome Following Egf Stimulation
In Skeletal Muscles
Co-Localizes With Mtmr12 In The Sarcomere
Alternative Antibody Names Anti-Myotubularin antibody
Anti-Phosphatidylinositol-3 -5-Bisphosphate 3-Phosphatase antibody
Anti-Phosphatidylinositol-3-Phosphate Phosphatase antibody
Anti-MTM1 antibody
Anti-CG2 antibody

Information sourced from Uniprot.org

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