• Western blot analysis of extracts of various cell lines, using MT-ND4 antibody (STJ119949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using MT-ND4 rabbit polyclonal antibody (STJ119949) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using MT-ND4 rabbit polyclonal antibody (STJ119949) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using MT-ND4 antibody (STJ119949) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MT-ND4 antibody (401-459) (STJ119949)

SKU:
STJ119949

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ND4 (401-459) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ND4
Gene ID: 4538
Uniprot ID: NU4M_HUMAN
Immunogen Region: 401-459
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 401-459 of human MT-ND4 (YP_002124311.1).
Immunogen Sequence: LVTALYSLYMFTTTQWGSLT HHINNMKPSFTRENTLMFMH LSPILLLSLNPDIITGFSS
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 4
Nadh Dehydrogenase Subunit 4
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 4 antibody
Anti-Nadh Dehydrogenase Subunit 4 antibody
Anti-MT-ND4 antibody
Anti-MTND4 antibody
Anti-NADH4 antibody
Anti-ND4 antibody

Information sourced from Uniprot.org

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