• Immunofluorescence analysis of HeLa cells using MT-ND2 Rabbit polyclonal antibody (STJ119947) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MT-ND2 in paraffin-embedded human colon carcinoma using MT-ND2 Rabbit polyclonal antibody (STJ119947) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MT-ND2 Rabbit polyclonal antibody (STJ119947) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-MT-ND2 antibody (248-347) (STJ119947)

SKU:
STJ119947

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ND2 (248-347) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ND2
Gene ID: 4536
Uniprot ID: NU2M_HUMAN
Immunogen Region: 248-347
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MT-ND2 (YP_003024027.1).
Immunogen Sequence: LLSLGGLPPLTGFLPKWAII EEFTKNNSLIIPTIMATITL LNLYFYLRLIYSTSITLLPM SNNVKMKWQFEHTKPTPFLP TLIALTTLLLPISPFMLMIL
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 2
Nadh Dehydrogenase Subunit 2
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 2 antibody
Anti-Nadh Dehydrogenase Subunit 2 antibody
Anti-MT-ND2 antibody
Anti-MTND2 antibody
Anti-NADH2 antibody
Anti-ND2 antibody

Information sourced from Uniprot.org

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