Western blot analysis of lysates from Mouse heart, using MT-CYB Rabbit pAb (STJ111820) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Mt-Cyb antibody (3-103) (STJ111820)

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STJ111820
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Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:Mt-Cyb
Gene ID:17711
Uniprot ID:CYB_MOUSE
Immunogen Region:3-103
Immunogen Sequence:NMRKTHPLFKIINHSFIDLP APSNISSWWNFGSLLGVCLM VQIITGLFLAMHYTSDTMTA FSSVTHICRDVNYGWLIRYM HANGASMFFICLFLHVGRGL Y
Specificity:A synthetic peptide corresponding to a sequence within amino acids 3-103 of mouse MT-CYB (NP_904340.1).
Function Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.
Protein Name Cytochrome B
Complex Iii Subunit 3
Complex Iii Subunit Iii
Cytochrome B-C1 Complex Subunit 3
Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit
Database Links Reactome: R-MMU-611105
Reactome: -MMU-9865881
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome B antibody
Anti-Complex Iii Subunit 3 antibody
Anti-Complex Iii Subunit Iii antibody
Anti-Cytochrome B-C1 Complex Subunit 3 antibody
Anti-Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit antibody
Anti-Mt-Cyb antibody
Anti-Cob antibody
Anti-Cytb antibody
Anti-mt-Cytb antibody
Anti-Mtcyb antibody

Information sourced from Uniprot.org

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