• Western blot analysis of extracts of various cell lines, using MT-CYB antibody (STJ111820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of Mouse heart, using MT-CYB Rabbit polyclonal antibody (STJ111820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MT-CYB antibody (STJ111820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Mt-Cyb antibody (3-103) (STJ111820)

SKU:
STJ111820

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mt-Cyb
Gene ID: 17711
Uniprot ID: CYB_MOUSE
Immunogen Region: 3-103
Specificity: A synthetic peptide corresponding to a sequence within amino acids 3-103 of mouse MT-CYB (NP_904340.1).
Immunogen Sequence: NMRKTHPLFKIINHSFIDLP APSNISSWWNFGSLLGVCLM VQIITGLFLAMHYTSDTMTA FSSVTHICRDVNYGWLIRYM HANGASMFFICLFLHVGRGL Y
Function Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.
Protein Name Cytochrome B
Complex Iii Subunit 3
Complex Iii Subunit Iii
Cytochrome B-C1 Complex Subunit 3
Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit
Database Links Reactome: R-MMU-611105
Reactome: -MMU-9865881
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome B antibody
Anti-Complex Iii Subunit 3 antibody
Anti-Complex Iii Subunit Iii antibody
Anti-Cytochrome B-C1 Complex Subunit 3 antibody
Anti-Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit antibody
Anti-Mt-Cyb antibody
Anti-Cob antibody
Anti-Cytb antibody
Anti-mt-Cytb antibody
Anti-Mtcyb antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance