• Western blot analysis of extracts of various cell lines, using MSRB3 antibody (STJ110312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using MSRB3 antibody (STJ110312) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using MSRB3 antibody (STJ110312) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MSRB3 antibody (STJ110312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MSRB3 antibody (1-185) (STJ110312)

SKU:
STJ110312

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSRB3 (1-185) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSRB3
Gene ID: 253827
Uniprot ID: MSRB3_HUMAN
Immunogen Region: 1-185
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human MSRB3 (NP_001026849.1).
Immunogen Sequence: MSAFNLLHLVTKSQPVALRA CGLPSGSCRDKKNCKVVFSQ QELRKRLTPLQYHVTQEKGT ESAFEGEYTHHKDPGIYKCV VCGTPLFKSETKFDSGSGWP SFHDVINSEAITFTDDFSYG MHRVETSCSQCGAHLGHIFD DGPRPTGKRYCINSAALSFT PADSSGTAEGGSGVASPAQA DKAEL
Tissue Specificity Widely expressed.
Function Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing.
Protein Name Methionine-R-Sulfoxide Reductase B3
Msrb3
Database Links Reactome: R-HSA-5676934
Cellular Localisation Isoform 1: Endoplasmic Reticulum
Isoform 2: Mitochondrion
Alternative Antibody Names Anti-Methionine-R-Sulfoxide Reductase B3 antibody
Anti-Msrb3 antibody
Anti-MSRB3 antibody
Anti-UNQ1965 antibody
Anti-PRO4487 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance