• Western blot analysis of extracts of various cell lines, using MSRA antibody (STJ28472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using MSRA antibody (STJ28472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using MSRA rabbit polyclonal antibody (STJ28472) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using MSRA rabbit polyclonal antibody (STJ28472) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using MSRA rabbit polyclonal antibody (STJ28472) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-MSRA antibody (24-235) (STJ28472)

SKU:
STJ28472

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSRA (24-235) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSRA
Gene ID: 4482
Uniprot ID: MSRA_HUMAN
Immunogen Region: 24-235
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-235 of human MSRA (NP_036463.1).
Immunogen Sequence: GNSASNIVSPQEALPGRKEQ TPVAAKHHVNGNRTVEPFPE GTQMAVFGMGCFWGAERKFW VLKGVYSTQVGFAGGYTSNP TYKEVCSEKTGHAEVVRVVY QPEHMSFEELLKVFWENHDP TQGMRQGNDHGTQYRSAIYP TSAKQMEAALSSKENYQKVL SEHGFGPITTDIREGQTFYY AEDYHQQYLSKNPNGYCGLG GTGVSCPVGIKK
Tissue Specificity Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
Function Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Protein Name Mitochondrial Peptide Methionine Sulfoxide Reductase
Peptide-Methionine
S-S-Oxide Reductase
Peptide Met(O Reductase
Protein-Methionine-S-Oxide Reductase
Pmsr
Database Links Reactome: R-HSA-5676934
Cellular Localisation Isoform 1: Mitochondrion
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Isoform 5: Cytoplasm
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Mitochondrial Peptide Methionine Sulfoxide Reductase antibody
Anti-Peptide-Methionine antibody
Anti-S-S-Oxide Reductase antibody
Anti-Peptide Met(O Reductase antibody
Anti-Protein-Methionine-S-Oxide Reductase antibody
Anti-Pmsr antibody
Anti-MSRA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance