• Human mesothelioma tissue was stained with Anti-Mesothelin (ABT-MSLN) Antibody
  • Human ovarian serous carcinoma tissue was stained with Anti-Mesothelin (ABT-MSLN) Antibody
  • Immunohistochemical analysis of paraffin-embedded Mesothelioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Ovarian Serous Carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Mesothelin (ABT-MSLN) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 69kDaObserved band size: 69kDa
  • Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Mesothelin (ABT-MSLN) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 69kDaObserved band size: 69kDa

Anti-MSLN antibody (400-500 aa) [ABT-MSLN] (STJ197037)

SKU:
STJ197037

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Mesothelin (400-500 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ABT-MSLN
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSLN
Gene ID: 10232
Uniprot ID: MSLN_HUMAN
Immunogen Region: 400-500 aa
Specificity: This antibody detects endogenous levels of human Mesothelin. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human Mesothelin at the amino acid range 400-500
Post Translational Modifications Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Function Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Protein Name Mesothelin
Cak1 Antigen
Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor
Mpf - Mesothelin - Cleaved Form
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Megakaryocyte-Potentiating Factor: Secreted
Isoform 3: Secreted
Alternative Antibody Names Anti-Mesothelin antibody
Anti-Cak1 Antigen antibody
Anti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibody
Anti-Mpf - Mesothelin - Cleaved Form antibody
Anti-MSLN antibody
Anti-MPF antibody

Information sourced from Uniprot.org

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