| Host: | Mouse |
| Applications: | WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Mesothelin (400-500 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ABT-MSLN |
| Conjugation: | Unconjugated |
| Isotype: | IgG2bk |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MSLN |
| Gene ID: | 10232 |
| Uniprot ID: | MSLN_HUMAN |
| Immunogen Region: | 400-500 aa |
| Specificity: | This antibody detects endogenous levels of human Mesothelin. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section |
| Immunogen: | Synthesized peptide derived from the human Mesothelin at the amino acid range 400-500 |
| Post Translational Modifications | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. |
| Protein Name | MesothelinCak1 AntigenPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating FactorMpf - Mesothelin - Cleaved Form |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusMegakaryocyte-Potentiating Factor: SecretedIsoform 3: Secreted |
| Alternative Antibody Names | Anti-Mesothelin antibodyAnti-Cak1 Antigen antibodyAnti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibodyAnti-Mpf - Mesothelin - Cleaved Form antibodyAnti-MSLN antibodyAnti-MPF antibody |
Information sourced from Uniprot.org