• Confocal imaging of U-2 OS cells using mSin3A Rabbit monoclonal antibody (STJ11103489, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using mSin3A Rabbit monoclonal antibody (STJ11103489, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded rat testis tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded rat spleen tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded rat colon tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded mouse testis tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded mouse colon tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded mouse brain tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded human small intestine tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of mSin3A in paraffin-embedded human esophagus tissue using mSin3A Rabbit monoclonal antibody (STJ11103489) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using mSin3A Rabbit monoclonal antibody (STJ11103489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-mSin3A antibody (1174-1273) [S9MR] (STJ11103489)

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STJ11103489

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-mSin3A (1174-1273) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIN3A
Gene ID: 25942
Uniprot ID: SIN3A_HUMAN
Immunogen Region: 1174-1273
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human mSin3A (Q96ST3).
Immunogen Sequence: YKMVYVIKSEDYMYRRTALL RAHQSHERVSKRLHQRFQAW VDKWTKEHVPREMAAETSKW LMGEGLEGLVPCTTTCDTET LHFVSINKYRVKYGTVFKAP
Tissue Specificity Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
Post Translational Modifications SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
Function Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.
Protein Name Paired Amphipathic Helix Protein Sin3a
Histone Deacetylase Complex Subunit Sin3a
Transcriptional Corepressor Sin3a
Database Links Reactome: R-HSA-3899300
Reactome: R-HSA-400206
Reactome: R-HSA-427413
Reactome: R-HSA-8936459
Reactome: R-HSA-9022538
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9615017
Reactome: R-HSA-9701898
Reactome: R-HSA-9707564
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Nucleolus
Recruited To The Nucleolus By Sap30l
Alternative Antibody Names Anti-Paired Amphipathic Helix Protein Sin3a antibody
Anti-Histone Deacetylase Complex Subunit Sin3a antibody
Anti-Transcriptional Corepressor Sin3a antibody
Anti-SIN3A antibody

Information sourced from Uniprot.org

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