• Western blot analysis of extracts of various cell lines, using Musashi-1 (MSI1) rabbit monoclonal antibody (STJ11102425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of U-2 OS cells using Musashi-1 (MSI1) Rabbit monoclonal antibody (STJ11102425) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Musashi-1 (MSI1) rabbit monoclonal antibody (STJ11102425) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Musashi-1 (MSI1) Rabbit monoclonal antibody (STJ11102425) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using Musashi-1 (MSI1) rabbit monoclonal antibody (STJ11102425) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Musashi-1 (MSI1) Rabbit monoclonal antibody (STJ11102425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-MSI1 antibody (1-100) [S5MR] (STJ11102425)

SKU:
STJ11102425

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Musashi 1/Msi1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSI1
Gene ID: 4440
Uniprot ID: MSI1H_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (O43347).
Immunogen Sequence: METDAPQPGLASPDSPHDPC KMFIGGLSWQTTQEGLREYF GQFGEVKECLVMRDPLTKRS RGFGFVTFMDQAGVDKVLAQ SRHELDSKTIDPKVAFPRRA
Tissue Specificity Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-GAU(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Protein Name Rna-Binding Protein Musashi Homolog 1
Musashi-1
Database Links Reactome: R-HSA-9010553
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Rna-Binding Protein Musashi Homolog 1 antibody
Anti-Musashi-1 antibody
Anti-MSI1 antibody

Information sourced from Uniprot.org

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