• Western blot analysis of extracts of 293T cells, using MRRF antibody (STJ11103493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using MRRF antibody (STJ11103493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MRRF antibody (163-262) [S3MR] (STJ11103493)

SKU:
STJ11103493

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MRRF (163-262) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRRF
Gene ID: 92399
Uniprot ID: RRFM_HUMAN
Immunogen Region: 163-262
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 163-262 of human MRRF (Q96E11).
Immunogen Sequence: RESGMNLNPEVEGTLIRVPI PQVTREHREMLVKLAKQNTN KAKDSLRKVRTNSMNKLKKS KDTVSEDTIRLIEKQISQMA DDTVAELDRHLAVKTKELLG
Function Responsible for the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with GFM2. Promotes mitochondrial ribosome recycling by dissolution of intersubunit contacts.
Protein Name Ribosome-Recycling Factor - Mitochondrial
Rrf
Mtrrf
Ribosome-Releasing Factor - Mitochondrial
Database Links Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Ribosome-Recycling Factor - Mitochondrial antibody
Anti-Rrf antibody
Anti-Mtrrf antibody
Anti-Ribosome-Releasing Factor - Mitochondrial antibody
Anti-MRRF antibody

Information sourced from Uniprot.org

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