| Short Description : | Rabbit polyclonal MRPL32 (19-188) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPL32 |
| Gene ID: | 64983 |
| Uniprot ID: | RM32_HUMAN |
| Immunogen Region: | 19-188 |
| Immunogen Sequence: | VLRNYWERLLRKLPQSRPGF PSPPWGPALAVQGPAMFTEP ANDTSGSKENSSLLDSIFWM AAPKNRRTIEVNRCRRRNPQ KLIKVKNNIDVCPECGHLKQ KHVLCAYCYEKVCKETAEIR RQIGKQEGGPFKAPTIETVV LYTGETPSEQDQGKRIIERD RKRPSWFTQN |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1). |
| Post Translational Modifications | MRPL32 precursor is processed by the m-AAA protease (composed of AFG3L2 and SPG7), which cleaves the N-terminal transit peptide. Cleavage by the m-AAA protease takes place prior to assembly into the large subunit, an essential step for mitochondrial ribosome (mitoribosome) assembly. Proper processing by the m-AAA protease is dependent on the zinc-binding region within the tightly folded C-terminal domain of MRPL32: zinc-dependent folding halts degradation initiated from the N-terminus and triggers the release of mature MRPL32. |
| Function | Component of the mitochondrial large ribosomal subunit (mt-LSU). The mitochondrial ribosome (mitoribosome) is a large ribonucleoprotein complex responsible for the synthesis of proteins inside mitochondria. |
| Protein Name | Large Ribosomal Subunit Protein Bl32m39s Ribosomal Protein L32 - MitochondrialL32mtMrp-L32 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl32m antibodyAnti-39s Ribosomal Protein L32 - Mitochondrial antibodyAnti-L32mt antibodyAnti-Mrp-L32 antibodyAnti-MRPL32 antibodyAnti-HSPC283 antibody |
Information sourced from Uniprot.org
