• Western blot analysis of extracts of various cell lines, using MRPL32 antibody (STJ112056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using MRPL32 rabbit polyclonal antibody (STJ112056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MRPL32 antibody (19-188) (STJ112056)

SKU:
STJ112056

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL32 (19-188) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL32
Gene ID: 64983
Uniprot ID: RM32_HUMAN
Immunogen Region: 19-188
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1).
Immunogen Sequence: VLRNYWERLLRKLPQSRPGF PSPPWGPALAVQGPAMFTEP ANDTSGSKENSSLLDSIFWM AAPKNRRTIEVNRCRRRNPQ KLIKVKNNIDVCPECGHLKQ KHVLCAYCYEKVCKETAEIR RQIGKQEGGPFKAPTIETVV LYTGETPSEQDQGKRIIERD RKRPSWFTQN
Protein Name Large Ribosomal Subunit Protein Bl32m
39s Ribosomal Protein L32 - Mitochondrial
L32mt
Mrp-L32
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl32m antibody
Anti-39s Ribosomal Protein L32 - Mitochondrial antibody
Anti-L32mt antibody
Anti-Mrp-L32 antibody
Anti-MRPL32 antibody
Anti-HSPC283 antibody

Information sourced from Uniprot.org

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