| Short Description : | Rabbit polyclonal Large Ribosomal Subunit Protein Ul13m (70-178) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:5000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MRPL13 |
| Gene ID: | 28998 |
| Uniprot ID: | RM13_HUMAN |
| Immunogen Region: | 70-178 |
| Immunogen Sequence: | NKWEQKVYSSHTGYPGGFRQ VTAAQLHLRDPVAIVKLAIY GMLPKNLHRRTMMERLHLFP DEYIPEDILKNLVEELPQPR KIPKRLDEYTQEEIDAFPRL WTPPEDYRL |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 70-178 of human MRPL13 (NP_054797.2). |
| Protein Name | Large Ribosomal Subunit Protein Ul13m39s Ribosomal Protein L13 - MitochondrialL13mtMrp-L13 |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Ul13m antibodyAnti-39s Ribosomal Protein L13 - Mitochondrial antibodyAnti-L13mt antibodyAnti-Mrp-L13 antibodyAnti-MRPL13 antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178111/370293/STJ11105467_1__76436.1713136227.jpg?c=1 "Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of MRPL13 in paraffin-embedded mouse lung tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178111/370294/STJ11105467_2__11799.1713136228.jpg?c=1 "Immunohistochemistry analysis of MRPL13 in paraffin-embedded mouse lung tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and MRPL13 knockdown (KD) HeLa cells using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178111/370295/STJ11105467_3__65223.1713136228.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL13 knockdown (KD) HeLa cells using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178111/370296/STJ11105467_4__25852.1713136229.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178111/370293/STJ11105467_1__76436.1713136227.jpg?c=1 "Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit polyclonal antibody (STJ11105467) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
