| Host: |
Mouse |
| Applications: |
FC/WB/IF/IHC |
| Reactivity: |
Baboon/Cat/Cow/Dog/Goat/Guinea Pig/Human/Horse/Monkey/Pig/Rabbit/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-MRP14/S100A9/calgranulin B is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
47-8D3 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow Cytometry (1-2 µg/million cells in 0, 1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 m |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: |
A BALB/c mouse was immunized with human peripheral blood monocyte components derived by affinity chromatography with Sepharose 4B coupled to rabbit monocyte antibodies. Fusion partner: NS-1. |
| Background | 47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
