• Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
  • 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0. 02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
  • Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
  • Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and  followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue).   Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Anti-MPO antibody (STJ13100487)

SKU:
STJ13100487-500

£337.50
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Host: NZ White Rabbit
Applications: IHC/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Nz White Rabbit polyclonal antibody anti-MPO is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Shipped as lyophilised. Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Purification: Ammonium sulphate precipitation
Dilution Range: A working concentration of 10-50 ug/ml is recommended. The optimal dilution should be determined by the end user. This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: MPO
Gene ID: 4353
Uniprot ID: PERM_HUMAN
Specificity: Specific for MPO.
Immunogen: Native myeloperoxidase isolated from human leucocytes
Protein Name Myeloperoxidase
Mpo Cleaved Into - Myeloperoxidase - 89 Kda Myeloperoxidase - 84 Kda Myeloperoxidase - Myeloperoxidase Light Chain - Myeloperoxidase Heavy Chain
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8941413
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Myeloperoxidase antibody
Anti-Mpo Cleaved Into - Myeloperoxidase - 89 Kda Myeloperoxidase - 84 Kda Myeloperoxidase - Myeloperoxidase Light Chain - Myeloperoxidase Heavy Chain antibody
Anti-MPO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance