• Western blot analysis of extracts of various cell lines, using MOV10 antibody (STJ24600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MOV10 antibody (1-310) (STJ24600)

SKU:
STJ24600

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MOV10 (1-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MOV10
Gene ID: 4343
Uniprot ID: MOV10_HUMAN
Immunogen Region: 1-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human MOV10 (NP_066014.1).
Immunogen Sequence: MPSKFSCRQLREAGQCFESF LVVRGLDMETDRERLRTIYN RDFKISFGTPAPGFSSMLYG MKIANLAYVTKTRVRFFRLD RWADVRFPEKRRMKLGSDIS KHHKSLLAKIFYDRAEYLHG KHGVDVEVQGPHEARDGQLL IRLDLNRKEVLTLRLRNGGT QSVTLTHLFPLCRTPQFAFY NEDQELPCPLGPGECYELHV HCKTSFVGYFPATVLWELLG PGESGSEGAGTFYIARFLA
Post Translational Modifications Ubiquitinated by the DCX(DCAF12) complex that specifically recognizes the glutamate-leucine (Glu-Leu) degron at the C-terminus, leading to its degradation. (Microbial infection) Cleaved and targeted for degradation by picornavirus proteases.
Function 5' to 3' RNA helicase that is involved in a number of cellular roles ranging from mRNA metabolism and translation, modulation of viral infectivity, inhibition of retrotransposition, or regulation of synaptic transmission. Plays an important role in innate antiviral immunity by promoting type I interferon production. Mechanistically, specifically uses IKKepsilon/IKBKE as the mediator kinase for IRF3 activation. Blocks HIV-1 virus replication at a post-entry step. Counteracts HIV-1 Vif-mediated degradation of APOBEC3G through its helicase activity by interfering with the ubiquitin-proteasome pathway. Inhibits also hepatitis B virus/HBV replication by interacting with HBV RNA and thereby inhibiting the early step of viral reverse transcription. Contributes to UPF1 mRNA target degradation by translocation along 3' UTRs. Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2. Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7. Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol. May function as a messenger ribonucleoprotein (mRNP) clearance factor. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.
Protein Name Helicase Mov-10
Armitage Homolog
Moloney Leukemia Virus 10 Protein
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-4086398
Reactome: R-HSA-5628897
Reactome: R-HSA-5687128
Reactome: R-HSA-8853884
Reactome: R-HSA-8934593
Reactome: R-HSA-8936459
Reactome: R-HSA-8943723
Reactome: R-HSA-8948700
Reactome: R-HSA-8986944
Reactome: R-HSA-9018519
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Reactome: R-HSA-9759811
Reactome: R-HSA-9768778
Cellular Localisation Cytoplasm
P-Body
Cytoplasmic Ribonucleoprotein Granule
Stress Granule
Nucleus
Co-Enriched In Cytoplasmic Foci With Tut4
In Developing Neurons
Localizes Both In Nucleus And Cytoplasm
But In The Adulthood It Is Only Cytoplasmic
After Infection
Relocalizes To The Denv Replication Complex In Perinuclear Regions
Alternative Antibody Names Anti-Helicase Mov-10 antibody
Anti-Armitage Homolog antibody
Anti-Moloney Leukemia Virus 10 Protein antibody
Anti-MOV10 antibody
Anti-KIAA1631 antibody

Information sourced from Uniprot.org

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