• Immunofluorescence – anti-mOrange antibody using hCEC cells transduced with mOrange-Rab5a; cells were fixed with methanol and anti-mOrange at 1/250
  • Anti-mOrange antibody at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-mOrange antibody using hCEC cells transduced with mOrange-Rab5a; cells were fixed with methanol and anti-mOrange at 1/250

Anti-mOrange antibody (STJ140227)

SKU:
STJ140227

£135.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/IEM
Reactivity: RFP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-mOrange is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: Epitope affinity purified
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:5000
IHC-F 1:50-1:500
IHC-P 1:50-1:500
IF 1:50-1:500
IEM 1:50-1:500
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Immunogen: Purified recombinant fluorescent protein produced in E. coli.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance