• STJ71692 (0. 5µg/ml) staining of Mouse Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71692 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ71692 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
  • STJ71692 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71692 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71692 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Monoglyceride Lipase antibody (Internal) (STJ71692)

SKU:
STJ71692-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Monoglyceride Lipase (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 35kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3kDa according to NP_
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MGLL
Gene ID: 11343
Uniprot ID: MGLL_HUMAN
Immunogen Region: Internal
Accession Number: NP_009214.1; NP_001003794.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
Immunogen Sequence: QDLPHLVNADGQY
Function Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
Protein Name Monoglyceride Lipase
Mgl
Hu-K5
Lysophospholipase Homolog
Lysophospholipase-Like
Monoacylglycerol Lipase
Magl
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-163560
Reactome: R-HSA-426048
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Monoglyceride Lipase antibody
Anti-Mgl antibody
Anti-Hu-K5 antibody
Anti-Lysophospholipase Homolog antibody
Anti-Lysophospholipase-Like antibody
Anti-Monoacylglycerol Lipase antibody
Anti-Magl antibody
Anti-MGLL antibody

Information sourced from Uniprot.org

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