Anti-MMP14 antibody (75-174) (STJ118137)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MMP14 (75-174) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MMP14 |
| Gene ID | 4323 |
| Uniprot ID | MMP14_HUMAN |
| Immunogen Region | 75-174 |
| Immunogen Sequence | QVTGKADADTMKAMRRPRCG VPDKFGAEIKANVRRKRYAI QGLKWQHNEITFCIQNYTPK VGEYATYEAIRKAFRVWESA TPLRFREVPYAYIREGHEKQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 75-174 of human MMP14/MT1-MMP (NP_004986.1). |
Additional Info
| Tissue Specificity | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. |
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK. |
| Function | Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis. Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL. |
| Protein Name | Matrix Metalloproteinase-14Mmp-14Mmp-X1Membrane-Type Matrix Metalloproteinase 1Mt-Mmp 1Mtmmp1Membrane-Type-1 Matrix MetalloproteinaseMt1-MmpMt1mmp |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-9839383Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMelanosomeCytoplasmIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvForms A Complex With Bst2 And Localizes To The Cytoplasm |
| Alternative Antibody Names | Anti-Matrix Metalloproteinase-14 antibodyAnti-Mmp-14 antibodyAnti-Mmp-X1 antibodyAnti-Membrane-Type Matrix Metalloproteinase 1 antibodyAnti-Mt-Mmp 1 antibodyAnti-Mtmmp1 antibodyAnti-Membrane-Type-1 Matrix Metalloproteinase antibodyAnti-Mt1-Mmp antibodyAnti-Mt1mmp antibodyAnti-MMP14 antibody |
Information sourced from Uniprot.org