| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MMACHC (63-282) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MMACHC |
| Gene ID: |
25974 |
| Uniprot ID: |
MMAC_HUMAN |
| Immunogen Region: |
63-282 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 63-282 of human MMACHC (NP_056321.2). |
| Immunogen Sequence: |
LKPFLQSCHLRMLTDPVDQC VAYHLGRVRESLPELQIEII ADYEVHPNRRPKILAQTAAH VAGAAYYYQRQDVEADPWGN QRISGVCIHPRFGGWFAIRG VVLLPGIEVPDLPPRKPHDC VPTRADRIALLEGFNFHWRD WTYRDAVTPQERYSEEQKAY FSTPPAQRLALLGLAQPSEK PSSPSPDLPFTTPAPKKPGN PSRARSWLSPRVSPPASPGP |
| Tissue Specificity | Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes. |
| Function | Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction. |
| Protein Name | Cyanocobalamin Reductase / Alkylcobalamin DealkylaseAlkylcobalamin -Glutathione S-AlkyltransferaseCblcCyanocobalamin ReductaseCyanide-EliminatingMethylmalonic Aciduria And Homocystinuria Type C ProteinMmachc |
| Database Links | Reactome: R-HSA-3359473Reactome: R-HSA-3359474Reactome: R-HSA-9759218 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Cyanocobalamin Reductase / Alkylcobalamin Dealkylase antibodyAnti-Alkylcobalamin -Glutathione S-Alkyltransferase antibodyAnti-Cblc antibodyAnti-Cyanocobalamin Reductase antibodyAnti-Cyanide-Eliminating antibodyAnti-Methylmalonic Aciduria And Homocystinuria Type C Protein antibodyAnti-Mmachc antibodyAnti-MMACHC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
