• Western blot analysis of extracts from wild type (WT) and MLKL knockout (KO) HeLa (KO) cells, using MLKL antibody (STJ11105002) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MLKL antibody (179-471) (STJ11105002)

SKU:
STJ11105002

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mixed Lineage Kinase Domain-Like Protein (179-471) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLKL
Gene ID: 197259
Uniprot ID: MLKL_HUMAN
Immunogen Region: 179-471
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 179-471 of human MLKL. (NP_689862.1).
Immunogen Sequence: YLPPKCMQEIPQEQIKEIKK EQLSGSPWILLRENEVSTLY KGEYHRAPVAIKVFKKLQAG SIAIVRQTFNKEIKTMKKFE SPNILRIFGICIDETVTPPQ FSIVMEYCELGTLRELLDRE KDLTLGKRMVLVLGAARGLY RLHHSEAPELHGKIRSSNFL VTQGYQVKLAGFELRKTQTS MSLGTTREKTDRVKSTAYLS PQELEDVFYQYDVKSEIYSF GIVLWEIATGDIPFQGCNS
Post Translational Modifications Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
Protein Name Mixed Lineage Kinase Domain-Like Protein
Hmlkl
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-9686347
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus
Localizes To The Cytoplasm And Translocates To The Plasma Membrane On Necroptosis Induction
Localizes To The Nucleus In Response To Orthomyxoviruses Infection
Alternative Antibody Names Anti-Mixed Lineage Kinase Domain-Like Protein antibody
Anti-Hmlkl antibody
Anti-MLKL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance