• STJ70213 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70213 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-MLH3 antibody (C-Term) (STJ70213)

SKU:
STJ70213-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MLH3 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei of HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MLH3
Gene ID: 27030
Uniprot ID: MLH3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001035197.1; NP_055196.2
Specificity: This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).
Immunogen Sequence: RQSLQQSMPPCEPP
Function Probably involved in the repair of mismatches in DNA.
Protein Name Dna Mismatch Repair Protein Mlh3
Mutl Protein Homolog 3
Database Links Reactome: R-HSA-912446
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Mlh3 antibody
Anti-Mutl Protein Homolog 3 antibody
Anti-MLH3 antibody

Information sourced from Uniprot.org

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