Anti-MLF1 antibody (1-268) (STJ115292)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MLF1 (1-268) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MLF1 |
| Gene ID | 4291 |
| Uniprot ID | MLF1_HUMAN |
| Immunogen Region | 1-268 |
| Immunogen Sequence | MFRMLNSSFEDDPFFSESIL AHRENMRQMIRSFSEPFGRD LLSISDGRGRAHNRRGHNDG EDSLTHTDVSSFQTMDQMVS NMRNYMQKLERNFGQLSVDP NGHSFCSSSVMTYSKIGDEP PKVFQASTQTRRAPGGIKET RKAMRDSDSGLEKMAIGHHI HDRAHVIKKSKNKKTGDEEV NQEFINMNESDAHAFDEEWQ SEVLKYKPGRHNLGNTRMRS VGHENPGSRELKRREKPQQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1). |
Additional Info
| Tissue Specificity | Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylation is required for binding to YWHAZ. |
| Function | Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. |
| Protein Name | Myeloid Leukemia Factor 1Myelodysplasia-Myeloid Leukemia Factor 1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusCell ProjectionCiliumCytoskeletonCilium Basal BodyShuttles Between The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Myeloid Leukemia Factor 1 antibodyAnti-Myelodysplasia-Myeloid Leukemia Factor 1 antibodyAnti-MLF1 antibody |
Information sourced from Uniprot.org