Anti-MKRN3 antibody (1-260) (STJ118513)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MKRN3 (1-260) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MKRN3 |
| Gene ID | 7681 |
| Uniprot ID | MKRN3_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MEEPAAPSEAHEAAGAQAGA EAAREGVSGPDLPVCEPSGE SAAPDSALPHAARGWAPFPV APVPAHLRRGGLRPAPASGG GAWPSPLPSRSSGIWTKQII CRYYIHGQCKEGENCRYSHD LSGRKMATEGGVSPPGASAG GGPSTAAHIEPPTQEVAEAP PAASSLSLPVIGSAAERGFF EAERDNADRGAAGGAGVESW ADAIEFVPGQPYRGRWVASA PEAPLQSSETERKQMAVGS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MKRN3 (NP_005655.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Acts as a key developmental timer that helps ensure puberty begins at the appropriate age, by inhibiting premature activation of the reproductive hormone cascade. Epigenetically regulates GNRH1 transcription by disrupting the binding of methyl-DNA binding protein 3/MBD3 to the promoter of GNRH1. Mechanistically, mediates the non-proteolytic ubiquitination of MBD3 at multiple sites with 'Lys27' ubiquitin linkages and thereby regulates the methylation status of the genome, including GNRH1 promoter. Modulates the stability and translation of GNRH1 mRNA by mediating the non-proteolytic ubiquitination of PABP family members PABPC1, PABPC3 and PABPC4 at multiple sites. Also participates in the maintenance of genomic and epigenomic stability by regulating the abundance of APEX2 via 'Lys-48'-linked ubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Makorin-3Ring Finger Protein 63Ring-Type E3 Ubiquitin Transferase Makorin-3Zinc Finger Protein 127 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Makorin-3 antibodyAnti-Ring Finger Protein 63 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Makorin-3 antibodyAnti-Zinc Finger Protein 127 antibodyAnti-MKRN3 antibodyAnti-D15S9 antibodyAnti-RNF63 antibodyAnti-ZNF127 antibody |
Information sourced from Uniprot.org