| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-MKI67 (1250-1300) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50-1:150 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | MKI67 |
| Gene ID: | 4288 |
| Uniprot ID: | KI67_HUMAN |
| Immunogen Region: | 1250-1300 |
| Specificity: | This antibody will detect both isoform 1 and isoform 2 in human. |
| Immunogen: | Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1. |
| Post Translational Modifications | Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA. |
| Function | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
| Protein Name | Proliferation Marker Protein Ki-67 Antigen Identified By Monoclonal Antibody Ki-67 Antigen Ki-67 Antigen Ki67 |
| Cellular Localisation | Chromosome Nucleus Nucleolus Associates With The Surface Of The Mitotic Chromosome The Perichromosomal Layer And Covers A Substantial Fraction Of The Mitotic Chromosome Surface Associates With Satellite Dna In G1 Phase Binds Tightly To Chromatin In Interphase Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes Predominantly Localized In The G1 Phase In The Perinucleolar Region In The Later Phases It Is Also Detected Throughout The Nuclear Interior Being Predominantly Localized In The Nuclear Matrix |
| Alternative Antibody Names | Anti-Proliferation Marker Protein Ki-67 antibody Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody Anti-Antigen Ki-67 antibody Anti-Antigen Ki67 antibody Anti-MKI67 antibody |
Information sourced from Uniprot.org