| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf/IP/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MK5/MAPKAPK5 (192-206) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. WB: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labe |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MAPKAPK5 |
| Gene ID: | 8550 |
| Uniprot ID: | MAPK5_HUMAN |
| Immunogen Region: | 192-206 |
| Accession Number: | NP_003659.2; NP_620777.1 |
| Immunogen Sequence: | PQVLEAQRRHQKEKS |
| Post Translational Modifications | Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated. |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Protein Name | Map Kinase-Activated Protein Kinase 5 Mapk-Activated Protein Kinase 5 Mapkap Kinase 5 Mapkap-K5 Mapkapk-5 Mk-5 Mk5 P38-Regulated/Activated Protein Kinase Prak |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-5687128 Reactome: R-HSA-6804756 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Phosphorylation And Activation Interaction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182 Activates The Protein Kinase Activity Followed By Translocation To The Cytoplasm Phosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export |
| Alternative Antibody Names | Anti-Map Kinase-Activated Protein Kinase 5 antibody Anti-Mapk-Activated Protein Kinase 5 antibody Anti-Mapkap Kinase 5 antibody Anti-Mapkap-K5 antibody Anti-Mapkapk-5 antibody Anti-Mk-5 antibody Anti-Mk5 antibody Anti-P38-Regulated/Activated Protein Kinase antibody Anti-Prak antibody Anti-MAPKAPK5 antibody Anti-PRAK antibody |
Information sourced from Uniprot.org