• STJ72942 (0. 5µg/ml) staining of MEF lysates (35µg protein in RIPA buffer) , from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
  • HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with STJ72942 (0. 5ug/ml) in the left panel and with STJ72943 (0. 5ug/ml) in the right panel.
  • STJ72942 and STJ72943 (1. 5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.

Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)

SKU:
STJ72942-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IP/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MK2/MAPKAPK2 (Internal) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MAPKAPK2
Gene ID: 9261
Uniprot ID: MAPK2_HUMAN
Immunogen Region: Internal
Accession Number: NP_004750.1; NP_116584.2
Specificity: This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Immunogen Sequence: SRVLKEDKERWED
Post Translational Modifications Sumoylation inhibits the protein kinase activity. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.
Function Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1.leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Protein Name Map Kinase-Activated Protein Kinase 2
Mapk-Activated Protein Kinase 2
Mapkap Kinase 2
Mapkap-K2
Mapkapk-2
Mk-2
Mk2
Database Links Reactome: R-HSA-171007
Reactome: R-HSA-199920
Reactome: R-HSA-2142691
Reactome: R-HSA-2559580
Reactome: R-HSA-3371453
Reactome: R-HSA-4420097
Reactome: R-HSA-450302
Reactome: R-HSA-450385
Reactome: R-HSA-450513
Reactome: R-HSA-5357905
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation And Subsequent Activation Releases The Autoinhibitory Helix
Resulting In The Export From The Nucleus Into The Cytoplasm
Alternative Antibody Names Anti-Map Kinase-Activated Protein Kinase 2 antibody
Anti-Mapk-Activated Protein Kinase 2 antibody
Anti-Mapkap Kinase 2 antibody
Anti-Mapkap-K2 antibody
Anti-Mapkapk-2 antibody
Anti-Mk-2 antibody
Anti-Mk2 antibody
Anti-MAPKAPK2 antibody

Information sourced from Uniprot.org

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