• Western blot analysis of 1) MCF7, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using MICU1 monoclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using MICU1 monoclonal antibody.
  • Immunofluorescence analysis of Human-appendix tissue. 1, MICU1 monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, MICU1 monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MICU1 antibody [Mix] (STJ97447)

SKU:
STJ97447

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Calcium uptake protein 1, mitochondrial is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: Mix
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution Range: WB 1:1000-2000
IHC 1:100-200
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MICU1
Gene ID: 10367
Uniprot ID: MICU1_HUMAN
Specificity: The antibody detects endogenous MICU1 protein.
Immunogen: Recombinant Protein of MICU1
Function Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake. Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening. Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.
Protein Name Calcium Uptake Protein 1 - Mitochondrial
Atopy-Related Autoantigen Calc
Ara Calc
Calcium-Binding Atopy-Related Autoantigen 1
Allergen Hom S 4
Database Links Reactome: R-HSA-8949215
Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Calcium Uptake Protein 1 - Mitochondrial antibody
Anti-Atopy-Related Autoantigen Calc antibody
Anti-Ara Calc antibody
Anti-Calcium-Binding Atopy-Related Autoantigen 1 antibody
Anti-Allergen Hom S 4 antibody
Anti-MICU1 antibody
Anti-CALC antibody
Anti-CBARA1 antibody

Information sourced from Uniprot.org

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