• Anti-MHC II DQ antibody [EBS-O-114] (STJ16101037)

Anti-MHC II DQ antibody [EBS-O-114] (STJ16101037)

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STJ16101037

£264.50 - £1,251.50
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Host: Mouse
Applications: FC/IF/IHC/IP
Reactivity: Human/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-MHC II DQ is suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: EBS-O-114
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (Formalin-fixed; 0, 5-1, 0 µg/ml for 30 minutes at RT; no special pretreatment is required for IHC staining of formalin/paraffin tissues). I
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: HLA-DQA2
Gene ID: 3118
Uniprot ID: DQA2_HUMAN
Immunogen: A BALB/c mouse was immunized with cells of T4+ CTL clone HG-38. Fusion partner: Sp2/0.
Tissue Specificity Restricted to skin Langerhans cells, although some expression at low levels may occur at the surface of B lymphoblastoid cells.
Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Protein Name Hla Class Ii Histocompatibility Antigen - Dq Alpha 2 Chain
Dx Alpha Chain
Hla Class Ii Histocompatibility Antigen - Dq(6 Alpha Chain
Hla-Dqa1
Mhc Class Ii Dqa2
Database Links Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-2132295
Reactome: R-HSA-389948
Reactome: R-HSA-877300
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Endosome Membrane
Lysosome Membrane
The Mhc Class Ii Complex Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway Until It Reaches The Cell Membrane For Antigen Presentation
Alternative Antibody Names Anti-Hla Class Ii Histocompatibility Antigen - Dq Alpha 2 Chain antibody
Anti-Dx Alpha Chain antibody
Anti-Hla Class Ii Histocompatibility Antigen - Dq(6 Alpha Chain antibody
Anti-Hla-Dqa1 antibody
Anti-Mhc Class Ii Dqa2 antibody
Anti-HLA-DQA2 antibody
Anti-HLA-DXA antibody

Information sourced from Uniprot.org

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