• Immunohistochemistry analysis of paraffin-embedded mouse heart using MGLL Rabbit polyclonal antibody (STJ28737) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MGLL Rabbit polyclonal antibody (STJ28737) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse heart, using MGLL antibody (STJ28737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using MGLL antibody (STJ28737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MGLL antibody (11-313) (STJ28737)

SKU:
STJ28737

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGLL (11-313) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGLL
Gene ID: 11343
Uniprot ID: MGLL_HUMAN
Immunogen Region: 11-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 11-313 of human MGLL (NP_009214.1).
Immunogen Sequence: MPEESSPRRTPQSIPYQDLP HLVNADGQYLFCRYWKPTGT PKALIFVSHGAGEHSGRYEE LARMLMGLDLLVFAHDHVGH GQSEGERMVVSDFHVFVRDV LQHVDSMQKDYPGLPVFLLG HSMGGAIAILTAAERPGHFA GMVLISPLVLANPESATTFK VLAAKVLNLVLPNLSLGPID SSVLSRNKTEVDIYNSDPLI CRAGLKVCFGIQLLNAVSRV ERALPKLTVPFLLLQGSAD
Tissue Specificity Detected in adipose tissue, lung, liver, kidney, brain and heart.
Function Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
Protein Name Monoglyceride Lipase
Mgl
Hu-K5
Lysophospholipase Homolog
Lysophospholipase-Like
Monoacylglycerol Lipase
Magl
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-163560
Reactome: R-HSA-426048
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Monoglyceride Lipase antibody
Anti-Mgl antibody
Anti-Hu-K5 antibody
Anti-Lysophospholipase Homolog antibody
Anti-Lysophospholipase-Like antibody
Anti-Monoacylglycerol Lipase antibody
Anti-Magl antibody
Anti-MGLL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance