Anti-MGEA5/OGA antibody [SRM14721] (STJ11109451)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
STJ11109451
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General Information

Short DescriptionRabbit monoclonal MGEA5/OGA antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSRM14721
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity purification
Dilution RangeWB, 1:3000-1:12000
IHC-P, 1:1000-1:5000
ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolOGA
Gene ID10724
Uniprot IDOGA_HUMAN
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.

Additional Info

Tissue Specificity Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
Post Translational Modifications Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other.cleavage does not decrease enzyme activity.
Function Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Protein Name Protein O-Glcnacase
Oga
Beta-N-Acetylglucosaminidase
Beta-N-Acetylhexosaminidase
Beta-Hexosaminidase
Meningioma-Expressed Antigen 5
N-Acetyl-Beta-D-Glucosaminidase
N-Acetyl-Beta-Glucosaminidase
Nuclear Cytoplasmic O-Glcnacase And Acetyltransferase
Ncoat
Database Links
Cellular Localisation Isoform 3: Nucleus
Isoform 1: Cytoplasm
Alternative Antibody Names Anti-Protein O-Glcnacase antibody
Anti-Oga antibody
Anti-Beta-N-Acetylglucosaminidase antibody
Anti-Beta-N-Acetylhexosaminidase antibody
Anti-Beta-Hexosaminidase antibody
Anti-Meningioma-Expressed Antigen 5 antibody
Anti-N-Acetyl-Beta-D-Glucosaminidase antibody
Anti-N-Acetyl-Beta-Glucosaminidase antibody
Anti-Nuclear Cytoplasmic O-Glcnacase And Acetyltransferase antibody
Anti-Ncoat antibody
Anti-OGA antibody
Anti-HEXC antibody
Anti-KIAA0679 antibody
Anti-MEA5 antibody
Anti-MGEA5 antibody

Information sourced from Uniprot.org

Citations

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