Anti-MGAT5 antibody (627-741) (STJ112582)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MGAT5 (627-741) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MGAT5 |
| Gene ID | 4249 |
| Uniprot ID | MGT5A_HUMAN |
| Immunogen Region | 627-741 |
| Immunogen Sequence | HGQVMWPPLSALQVKLAEPG QSCKQVCQESQLICEPSFFQ HLNKDKDMLKYKVTCQSSEL AKDILVPSFDPKNKHCVFQG DLLLFSCAGAHPRHQRVCPC RDFIKGQVALCKDCL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 627-741 of human GNT-V/MGAT5 (NP_002401.1). |
Additional Info
| Post Translational Modifications | N-glycosylated. A secreted form is released from the membrane after cleavage by gamma-secretase. |
| Function | Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2. Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation. Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis. Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A: Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix. |
| Protein Name | Alpha-1 -6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase AAlpha-Mannoside Beta-1 -6-N-Acetylglucosaminyltransferase VGlcnac-T VGnt-VMannoside Acetylglucosaminyltransferase 5N-Acetylglucosaminyl-Transferase V Cleaved Into - Secreted Alpha-1 -6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase ASecreted Beta-1 -6-N-Acetylglucosaminyltransferase VSecreted Gnt-V |
| Database Links | Reactome: R-HSA-9694548Reactome: R-HSA-975577 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinSecreted Alpha-16-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase A: Secreted |
| Alternative Antibody Names | Anti-Alpha-1 -6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase A antibodyAnti-Alpha-Mannoside Beta-1 -6-N-Acetylglucosaminyltransferase V antibodyAnti-Glcnac-T V antibodyAnti-Gnt-V antibodyAnti-Mannoside Acetylglucosaminyltransferase 5 antibodyAnti-N-Acetylglucosaminyl-Transferase V Cleaved Into - Secreted Alpha-1 -6-Mannosylglycoprotein 6-Beta-N-Acetylglucosaminyltransferase A antibodyAnti-Secreted Beta-1 -6-N-Acetylglucosaminyltransferase V antibodyAnti-Secreted Gnt-V antibodyAnti-MGAT5 antibodyAnti-GGNT5 antibody |
Information sourced from Uniprot.org