| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Mesothelin (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 68.1kDa according to NP_005814.2 and of 69.0kDa according to NP_037536.2). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MSLN |
| Gene ID: | 10232 |
| Uniprot ID: | MSLN_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005814.2; NP_037536.2 |
| Specificity: | This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2). |
| Immunogen Sequence: | CPSGKKAREIDES |
| Post Translational Modifications | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. |
| Protein Name | MesothelinCak1 AntigenPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating FactorMpf - Mesothelin - Cleaved Form |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusMegakaryocyte-Potentiating Factor: SecretedIsoform 3: Secreted |
| Alternative Antibody Names | Anti-Mesothelin antibodyAnti-Cak1 Antigen antibodyAnti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibodyAnti-Mpf - Mesothelin - Cleaved Form antibodyAnti-MSLN antibodyAnti-MPF antibody |
Information sourced from Uniprot.org