• Western blot analysis of extracts of MERS-CoV Nucleoprotein, using MERS-CoV Nucleoprotein antibody (STJ11103394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-MERS-CoV Nucleoprotein antibody (1-413) (STJ11103394)

SKU:
STJ11103394

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Host: Rabbit
Applications: WB
Reactivity: MERS-CoV
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MERS-CoV Nucleoprotein (1-413) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-413
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-413 of coronavirus Nucleoprotein (YP_009047211.1).
Immunogen Sequence: MASPAAPRAVSFADNNDITN TNLSRGRGRNPKPRAAPNNT VSWYTGLTQHGKVPLTFPPG QGVPLNANSTPAQNAGYWRR QDRKINTGNGIKQLAPRWYF YYTGTGPEAALPFRAVKDGI VWVHEDGATDAPSTFGTRNP NNDSAIVTQFAPGTKLPKNF HIEGTGGNSQSSSRASSLSR NSSRSSSQGSRSGNSTRGTS PGPSGIGAVGGDLLYLDLLN RLQALESGKVKQSQPKVIT
Background The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH (4) , is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia.

Information sourced from Uniprot.org

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