• Western blot analysis of extracts of various cell lines, using MEPE antibody (STJ11103665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using MEPE Rabbit polyclonal antibody (STJ11103665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MEPE antibody (90-256) (STJ11103665)

SKU:
STJ11103665

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MEPE (90-256) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEPE
Gene ID: 56955
Uniprot ID: MEPE_HUMAN
Immunogen Region: 90-256
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-256 of human MEPE (NP_064588.1).
Immunogen Sequence: FTNRQRLNKEYSISNKENTH NGLRMSIYPKSTGNKGFEDG DDAISKLHDQEEYGAALIRN NMQHIMGPVTAIKLLGEENK ENTPRNVLNIIPASMNYAKA HSKDKKKPQRDSQAQKSPVK SKSTHRIQHNIDYLKHLSKV KKIPSDFEGSGYTDLQERGD NDISPFS
Tissue Specificity Expressed by osteoblasts. Expressed by stem cells in dental pulp. Expressed by mesenchymal cells in dental papilla and dental pulp. Expressed in teeth, specifically in decidious dentin. Expressed in ondotoblasts. Expressed in salivary glands. Secreted from oncogenic hypophosphatemic tumors.
Post Translational Modifications Phosphorylated on serine residues in the ASARM motif (in vitro) by FAM20C.the phosphorylation is important for the inhibition of bone mineralization. Cleaved by CTSB/cathepsin B.the cleavage is blocked by metalloprotease PHEX.
Function Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage. Promotes dental pulp stem cell proliferation and differentiation.
Protein Name Matrix Extracellular Phosphoglycoprotein
Osteoblast/Osteocyte Factor 45
Of45
Osteoregulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Extracellular Phosphoglycoprotein antibody
Anti-Osteoblast/Osteocyte Factor 45 antibody
Anti-Of45 antibody
Anti-Osteoregulin antibody
Anti-MEPE antibody

Information sourced from Uniprot.org

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