• Western blot analysis of various lysates using MED19 Rabbit polyclonal antibody (STJ119860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MED19 antibody (60-160) (STJ119860)

SKU:
STJ119860

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MED19 (60-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MED19
Gene ID: 219541
Uniprot ID: MED19_HUMAN
Immunogen Region: 60-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-160 of human MED19 (NP_703151.1).
Immunogen Sequence: AGCGPFYLMRELPGSTELTG STNLITHYNLEQAYNKFCGK KVKEKLSNFLPDLPGMIDLP GSHDNSSLRSLIEKPPILSS SFNPITGTMLAGFRLHTGPL P
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protein Name Mediator Of Rna Polymerase Ii Transcription Subunit 19
Lung Cancer Metastasis-Related Protein 1
Mediator Complex Subunit 19
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 19 antibody
Anti-Lung Cancer Metastasis-Related Protein 1 antibody
Anti-Mediator Complex Subunit 19 antibody
Anti-MED19 antibody
Anti-LCMR1 antibody

Information sourced from Uniprot.org

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