Anti-MED15 antibody (510-590 aa) (STJ190945)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 510-590
General Information
| Short Description | Rabbit polyclonal anti-Mediator of RNA polymerase II transcription subunit 15 (510-590 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MED15 |
| Gene ID | 51586 |
| Uniprot ID | MED15_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 510-590 |
| Immunogen Region | 510-590 aa |
| Specificity | MED15 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by TRIM11, leading to proteasomal degradation. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 15Activator-Recruited Cofactor 105 Kda ComponentArc105Ctg Repeat Protein 7aMediator Complex Subunit 15Positive Cofactor 2 Glutamine/Q-Rich-Associated ProteinPc2 Glutamine/Q-Rich-Associated ProteinTpa-Inducible Gene 1 ProteinTig-1Trinucleotide Repeat-Containing Gene 7 Protein |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-212436Reactome: R-HSA-381340Reactome: R-HSA-9833110 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 15 antibodyAnti-Activator-Recruited Cofactor 105 Kda Component antibodyAnti-Arc105 antibodyAnti-Ctg Repeat Protein 7a antibodyAnti-Mediator Complex Subunit 15 antibodyAnti-Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein antibodyAnti-Pc2 Glutamine/Q-Rich-Associated Protein antibodyAnti-Tpa-Inducible Gene 1 Protein antibodyAnti-Tig-1 antibodyAnti-Trinucleotide Repeat-Containing Gene 7 Protein antibodyAnti-MED15 antibodyAnti-ARC105 antibodyAnti-CTG7A antibodyAnti-PCQAP antibodyAnti-TIG1 antibodyAnti-TNRC7 antibody |
Information sourced from Uniprot.org