Anti-MECR antibody (54-373) (STJ11105571)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Enoyl-Acyl-Carrier-Protein Reductase-Mitochondrial (54-373) antibody for use in IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MECR |
| Gene ID | 51102 |
| Uniprot ID | MECR_HUMAN |
| Immunogen Region | 54-373 |
| Immunogen Sequence | PAKVVELKNLELAAVRGSDV RVKMLAAPINPSDINMIQGN YGFLPELPAVGGNEGVAQVV AVGSNVTGLKPGDWVIPANA GLGTWRTEAVFSEEALIQVP SDIPLQSAATLGVNPCTAYR MLMDFEQLQPGDSVIQNASN SGVGQAVIQIAAALGLRTIN VVRDRPDIQKLSDRLKSLGA EHVITEEELRRPEMKNFFKD MPQPRLALNCVGGKSSTELL RQLARGGTMVTYGGMAKQP |
| Specificity | Recombinant Protein corresponding to a sequence within amino acids 54-373 of human MECR (NP_057095.4). |
Additional Info
| Tissue Specificity | Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas. Weakly or not expressed in lung. |
| Function | Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates. May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells. Involved in iron homeostasis.affecting Fe-S cluster assembly and ceramide metabolism. Required for proper morphology and bioenergetic functions of mitochondria. Required for maintenance of neurons. |
| Protein Name | Enoyl-Acyl-Carrier-Protein Reductase - Mitochondrial2-Enoyl Thioester ReductaseNuclear Receptor-Binding Factor 1Hsnrbf-1Nrbf-1 |
| Database Links | Reactome: R-HSA-77346 |
| Cellular Localisation | Isoform 1: MitochondrionIsoform 2: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Enoyl-Acyl-Carrier-Protein Reductase - Mitochondrial antibodyAnti-2-Enoyl Thioester Reductase antibodyAnti-Nuclear Receptor-Binding Factor 1 antibodyAnti-Hsnrbf-1 antibodyAnti-Nrbf-1 antibodyAnti-MECR antibodyAnti-NBRF1 antibodyAnti-CGI-63 antibody |
Information sourced from Uniprot.org