| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-MeCP2 isoform 1 (1-50) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:2, 500-1:5, 000 ELISA: 1:50, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Mecp2 |
| Gene ID: | 17257 |
| Uniprot ID: | MECP2_MOUSE |
| Immunogen Region: | 1-50 |
| Immunogen: | Synthetic peptide taken within amino acid region 1-50 on mouse Methyl-CpG-binding Protein 2 isoform 1 protein. |
| Post Translational Modifications | Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2 Mecp-2 Protein Mecp2 |
| Database Links | Reactome: R-MMU-9022692 Q9Z2D6-1 |
| Cellular Localisation | Nucleus Colocalized With Methyl-Cpg In The Genome Colocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibody Anti-Mecp-2 Protein antibody Anti-Mecp2 antibody Anti-Mecp2 antibody |
Information sourced from Uniprot.org