• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from RAW264.7 and NIH/3T3 cells, using ME3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody

Anti-ME3 antibody (545-594 aa) (STJ94060)

SKU:
STJ94060

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NADP-dependent malic enzyme, mitochondrial (545-594 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ME3
Gene ID: 10873
Uniprot ID: MAON_HUMAN
Immunogen Region: 545-594 aa
Specificity: ME3 Polyclonal Antibody detects endogenous levels of ME3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ME3 at the amino acid range 545-594
Function Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor. Can also reverse the decarboxylation reaction, but only with significantly lower efficiency.
Protein Name Nadp-Dependent Malic Enzyme - Mitochondrial
Nadp-Me
Malic Enzyme 3
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nadp-Dependent Malic Enzyme - Mitochondrial antibody
Anti-Nadp-Me antibody
Anti-Malic Enzyme 3 antibody
Anti-ME3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance