• Immunofluorescence analysis of L929 cells using ME3 Rabbit polyclonal antibody (STJ118232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ME3 in paraffin-embedded Human colon carcinoma using ME3 Rabbit polyclonal antibody (STJ118232) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ME3 in paraffin-embedded Rat ovary using ME3 Rabbit polyclonal antibody (STJ118232) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ME3 in paraffin-embedded Mouse heart using ME3 Rabbit polyclonal antibody (STJ118232) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ME3 Rabbit polyclonal antibody (STJ118232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ME3 antibody (505-604) (STJ118232)

SKU:
STJ118232

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ME3 (505-604) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ME3
Gene ID: 10873
Uniprot ID: MAON_HUMAN
Immunogen Region: 505-604
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 505-604 of human ME3 (NP_001014811.1).
Immunogen Sequence: IRHIPDEIFLLTAEQIAQEV SEQHLSQGRLYPPLSTIRDV SLRIAIKVLDYAYKHNLASY YPEPKDKEAFVRSLVYTPDY DSFTLDSYTWPKEAMNVQTV
Tissue Specificity Expressed predominantly in organs with a low-division rate.
Function Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor. Can also reverse the decarboxylation reaction, but only with significantly lower efficiency.
Protein Name Nadp-Dependent Malic Enzyme - Mitochondrial
Nadp-Me
Malic Enzyme 3
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nadp-Dependent Malic Enzyme - Mitochondrial antibody
Anti-Nadp-Me antibody
Anti-Malic Enzyme 3 antibody
Anti-ME3 antibody

Information sourced from Uniprot.org

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