| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MDC1 |
| Gene ID: |
9656 |
| Uniprot ID: |
MDC1_HUMAN |
| Immunogen Region: |
1-350 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2). |
| Immunogen Sequence: |
MEDTQAIDWDVEEEEETEQS SESLRCNVEPVGRLHIFSGA HGPEKDFPLHLGKNVVGRMP DCSVALPFPSISKQHAEIEI LAWDKAPILRDCGSLNGTQI LRPPKVLSPGVSHRLRDQEL ILFADLLCQYHRLDVSLPFV SRGPLTVEETPRVQGETQPQ RLLLAEDSEEEVDFLSERRM VKKSRTTSSSVIVPESDEEG HSPVLGGLGPPFAFNLNSDT DVEEGQQPATEEASSAARR |
| Tissue Specificity | Highly expressed in testis. |
| Post Translational Modifications | Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain. Phosphorylated at Ser-168 and Ser-196 by CK2 in response to DNA damage during mitosis, promoting interaction with TOPBP1. Phosphorylated by CK2 in response to DNA damage, promoting interaction with NBN and recruitment of the MRN complex to DNA damage sites. Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation. Ubiquitinated by RNF4, leading to proteasomal degradation.undergoes 'Lys-48'-linked polyubiquitination. |
| Function | Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs): TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis. Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs. |
| Protein Name | Mediator Of Dna Damage Checkpoint Protein 1Nuclear Factor With Brct Domains 1 |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-6796648Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusChromosomeAssociated With ChromatinRelocalizes To Discrete Nuclear Foci Following Dna DamageThis Requires 'Ser-139' Phosphorylation Of H2axColocalizes With Aptx At Sites Of Dna Double-Strand Breaks |
| Alternative Antibody Names | Anti-Mediator Of Dna Damage Checkpoint Protein 1 antibodyAnti-Nuclear Factor With Brct Domains 1 antibodyAnti-MDC1 antibodyAnti-KIAA0170 antibodyAnti-NFBD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
