| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MCU |
| Gene ID: |
90550 |
| Uniprot ID: |
MCU_HUMAN |
| Immunogen Region: |
51-351 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 51-170 of human MCU (NP_612366.1). |
| Immunogen Sequence: |
VHQRIASWQNLGAVYCSTVV PSDDVTVVYQNGLPVISVRL PSRRERCQFTLKPISDSVGV FLRQLQEEDRGIDRVAIYSP DGVRVAASTGIDLLLLDDFK LVINDLTYHVRPPKRDLLSH |
| Post Translational Modifications | Phosphorylation by CaMK2 in heart leads to increased MCU current. The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results. Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization. Glutathionylation at Cys-97 in response to reactive oxygen species (ROS) promotes MCU higher-order assembly, leading to constitutive activation of the MCU channel and mitochondrial calcium overload. Undergoes proteolytic degradation by SPG7. |
| Function | Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix. MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. Participates in mitochondrial permeability transition during ischemia-reperfusion injury. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in antigen processing and presentation. |
| Protein Name | Calcium Uniporter Protein - MitochondrialHsmcuCoiled-Coil Domain-Containing Protein 109a |
| Database Links | Reactome: R-HSA-8949215Reactome: R-HSA-8949664 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Calcium Uniporter Protein - Mitochondrial antibodyAnti-Hsmcu antibodyAnti-Coiled-Coil Domain-Containing Protein 109a antibodyAnti-MCU antibodyAnti-C10orf42 antibodyAnti-CCDC109A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
