• Western blot analysis of extracts of various cell lines, using MCPH1 antibody (STJ27425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MCPH1 antibody (263-562) (STJ27425)

SKU:
STJ27425

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCPH1
Gene ID: 79648
Uniprot ID: MCPH1_HUMAN
Immunogen Region: 263-562
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human MCPH1 (NP_001166046.1).
Immunogen Sequence: VTPDQKQAAGMSQETFEEKY RLSPTLSSTKGHLLIHSRPR SSSVKRKRVSHGSHSPPKEK CKRKRSTRRSIMPRLQLCRS EDRLQHVAGPALEALSCGES SYDDYFSPDNLKERYSENLP PESQLPSSPAQLSCRSLSKK ERTSIFEMSDFSCVGKKTRT VDITNFTAKTISSPRKTGNG EGRATSSCVTSAPEEALRCC RQAGKEDACPEGNGFSYTIE DPALPKGHDDDLTPLEGSL
Tissue Specificity Expressed in fetal brain, liver and kidney.
Function Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
Protein Name Microcephalin
Database Links Reactome: R-HSA-2299718
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-Microcephalin antibody
Anti-MCPH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance