• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g MCM7 antibody (STJ24528). Western blot was performed from the immunoprecipitate using MCM7 antibody (STJ24528) at a dilution of 1:500.
  • Immunofluorescence analysis of U-2 OS cells using MCM7 Rabbit polyclonal antibody (STJ24528) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using (STJ24528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MCM7 antibody (1-140) (STJ24528)

SKU:
STJ24528

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCM7 (1-140) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM7
Gene ID: 4176
Uniprot ID: MCM7_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MCM7 (NP_005907.3).
Immunogen Sequence: MALKDYALEKEKVKKFLQEF YQDDELGKKQFKYGNQLVRL AHREQVALYVDLDDVAEDDP ELVDSICENARRYAKLFADA VQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVR SPQNQYPAELMRRFELYFQG
Post Translational Modifications O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. Ubiquitinated by ECS(LRR1) E3 ubiquitin-protein ligase complex when forks converge following formation of DNA interstrand cross-links. During mitosis, ubiquitinated by TRAIP when forks converge following formation of DNA interstrand cross-links. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase.
Function Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Protein Name Dna Replication Licensing Factor Mcm7
Cdc47 Homolog
P1.1-Mcm3
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-176974
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses
Alternative Antibody Names Anti-Dna Replication Licensing Factor Mcm7 antibody
Anti-Cdc47 Homolog antibody
Anti-P1.1-Mcm3 antibody
Anti-MCM7 antibody
Anti-CDC47 antibody
Anti-MCM2 antibody

Information sourced from Uniprot.org

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