What is mCherry-tag
This monomeric red fluorescent protein is a member of the mFruits family, which is derived from DsRed originating from Discosoma sea anemones. It is used for tagging cell components in fluorescence spectroscopy and microscopy, as well as bacteria labelling.
This fluorescent protein chromophore is extensively used in genome editing by selectively inserting it into the genetic material of a wide array of organisms.
mCherry-tag is excited by light between 540 and 590 nm and emits light ranging from 550 to 650 nm.
mCherry-tag structure
This tetrameric protein is made up from 236 residues and has a mass of 26.722 kDa, and the gene that encodes for it is 711bp long.
The crystal structure of mCherry-tag consists of 3 alpha helices and 13 beta sheets, that create the beta barrel.
The mCherry-tag's chromophore consits of methionine, tyrosine, and glycine, which are post-translationally changed to imidazolinone. The number of these residues in sequence are 71, 72, and 73.
The extended pi-electron conjugation is the reason for mCherry's red-shifted absorbance and emission.
mCherry-tag Monoclonal antibodies
Mouse monoclonal antibody anti-mCherry is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
mCherry-tag Polyclonal antibodies
Goat polyclonal antibody anti-RFP is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Goat polyclonal antibody anti-Red Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.