Anti-mCherry antibody (Full-Length) [1C51] (STJA0040771)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenRecombinant full length mCherry expressed and purified from E. coli.
General Information
| Short Description | Mouse monoclonal anti-mCherry (Full-Length) for use in ICC and WB in Species Independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1C51 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:1000-1:2000ICC 1:250-1:500 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 ยตL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20ยฐC for |
Target Information
| Immunogen | Recombinant full length mCherry expressed and purified from E. coli. |
| Immunogen Region | Full-Length |
| Immunogen Sequence | Species Independent |
| Specificity | The antibody reacts with a band at ~28 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry. |
Additional Info
| Background | Our Anti-mCherry mouse monoclonal primary antibody detects species independent mCherry, and is igg. It is validated for use in ICC, WB. |
Information sourced from Uniprot.org