| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | MAPKAPK5 |
| Gene ID: | 8550 |
| Uniprot ID: | MAPK5_HUMAN |
| Immunogen Region: | 302-471 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 302-471 of human MAPKAPK5 (NP_003659.2). |
| Immunogen Sequence: | PWLNSTEALDNVLPSAQLMM DKAVVAGIQQAHAEQLANMR IQDLKVSLKPLHSVNNPILR KRKLLGTKPKDSVYIHDHEN GAEDSNVALEKLRDVIAQCI LPQAGENEDEKLNEVMQEAW KYNRECKLLRDTLQSFSWNG RGFTDKVDRLKLAEIVKQVI EEQTTSHESQ |
| Tissue Specificity | Expressed ubiquitously. |
| Post Translational Modifications | Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4.which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated. |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Protein Name | Map Kinase-Activated Protein Kinase 5 Mapk-Activated Protein Kinase 5 Mapkap Kinase 5 Mapkap-K5 Mapkapk-5 Mk-5 Mk5 P38-Regulated/Activated Protein Kinase Prak |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-5687128 Reactome: R-HSA-6804756 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Phosphorylation And Activation Interaction With Erk3/Mapk6 Or Erk4/Mapk4 And Phosphorylation At Thr-182 Activates The Protein Kinase Activity Followed By Translocation To The Cytoplasm Phosphorylation By Pka/Prkaca At Ser-115 Also Induces Nuclear Export |
| Alternative Antibody Names | Anti-Map Kinase-Activated Protein Kinase 5 antibody Anti-Mapk-Activated Protein Kinase 5 antibody Anti-Mapkap Kinase 5 antibody Anti-Mapkap-K5 antibody Anti-Mapkapk-5 antibody Anti-Mk-5 antibody Anti-Mk5 antibody Anti-P38-Regulated/Activated Protein Kinase antibody Anti-Prak antibody Anti-MAPKAPK5 antibody Anti-PRAK antibody |
Information sourced from Uniprot.org